Coding

Part:BBa_K1618010:Design

Designed by: Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-08-13)


pSB1C3-GlgX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2211
    Illegal EcoRI site found at 1329
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1329
    Illegal SpeI site found at 2212
    Illegal PstI site found at 2226
    Illegal NotI site found at 7
    Illegal NotI site found at 2219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1329
    Illegal BamHI site found at 77
    Illegal BamHI site found at 1285
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2212
    Illegal EcoRI site found at 1329
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1329
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2212
    Illegal PstI site found at 2226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 711


Design Notes

None


Source

Bacterial sequence.

References